effect of fruit essential oil on ephedrine induced manic like behavior: evidence from a new protocol

introduction: estimating ephedrine’s effect on animal’s locomotor activity and evaluation of cumin fruit essential oil (feo) effect and mechanism of ephedrine-induced hyper-locomotion. methods: a new protocol developed for evaluation of manic behavior induced by ephedrine in a dose and time dependent manner. following the suppressive effects of cumin feo on acquisition and expression of ephedrine induced manic behavior was examined. furthermore, the effect of l-name on expression of manic behavior and effect of bicuculline on suppressive effects of cumin feo was evaluated. results: ephedrine at the dose of 100 mg/kg (i.p.) in a five days protocol significantly increased the locomotor activity in mice. in addition, cumin feo at the specific dose of 2% significantly suppressed the acquisition and expression of hyperactivity induced by ephedrine. l-name at doses of 30 (p<0.01) and 40 mg/kg (p<0.05) showed similar effects as cumin feo at the dose of 2%. bicuculline (as a gabaa antagonist) at the specific dose of 2 mg/kg (p<0.01) could significantly reverse the suppressive effects of cumin at the dose of 2%. conclusion: it could be concluded that cumin can suppress the manic behavior induced by ephedrine through the both previously suggested mechanisms of nitric oxide syntheses pathway and gabaergic system. declaration of interest: none. keywords : cuminum cyminum fruit, ephedrine, manic disorder.